Senior Research Associate, PK/PD Assays

NewLimit is seeking a Senior Research Associate with strong experience in molecular biology and translational biomarker assay development to join our team. This role will contribute to the development and execution of pharmacokinetic (PK) and pharmacodynamic (PD) assays to support our therapeutic programs. You will work closely with cross-functional teams to develop and implement molecular assays that quantify the biodistribution of our mRNA-LNP medicines and the biological response to them in preclinical studies.

• →Develop, validate, and execute PK and PD biomarker assays to support preclinical studies and therapeutic discovery programs
• →Design and perform molecular biology assays (e.g., qPCR, RT-qPCR, ddPCR, NGS-based assays, ELISA) to quantify nucleic acids, gene and protein expression in biological samples
• →Contribute to experimental sample processing and preparation workflows including animal dissections, tissue harvesting, and primary cell culture
• →Collaborate with our Epigenetic Editing team to perform large-scale, single cell perturbation experiments
• →Perform state-of-the-art single cell profiling experiments to map epigenetic features of aging

• Bachelor’s degree in molecular biology, cell biology, biochemistry, or a related scientific field
• 2+ years of laboratory experience in molecular biology and quantitative assay development (e.g., qPCR, RT-qPCR, ddPCR, ELISA, NGS-based)
• Hands-on experience with primary cell culture, tissue harvesting and complex biological matrices for molecular assays
• Familiarity with bioanalytical assay qualification/validation principles for PK/PD biomarker measurements
• Understanding of data quality, documentation, and experimental practices aligned with preclinical regulatory expectations (e.g., GLP-like practices or bioanalytical method validation guidelines)

• Experience working in environments following GLP or regulated bioanalytical workflows supporting preclinical or IND-enabling studies
• Experience with flow cytometry sorting
• Experience with NGS methods